What is the Suresign UTI Test Kit?
The Suresign UTI test kit provides a qualitative analysis of Leukocytes and Nitrites in human urine, aiding in the accurate detection of urinary tract infections.
The test kit contains 3 individual tests that include a plastic strip that is affixed with two separate reagent areas, one for Nitrates and one for Leukocytes. The test is a single-use test that is intended for self-testing.
How does the Suresign UTI Test work?
The reagents found in the plastic strips react to the contents found in the urine and change accordingly to the sample:
For Leukocyte detection, the test reveals the presence of enzymes known as granulocyte esterases. These enzymes break down a special type of chemical called derivatized pyrazole amino acid esters to release a related but simpler chemical called derivatized hydroxyl pyrazole. This pyrazole chemical then reacts with a diazonium salt to produce a beige-pink to purple colour, indicating the presence of Leukocytes.
For Nitrate detection, the test looks at how certain bacteria in urine change nitrate to a related substance called nitrite. In an acidic medium, nitrite in the urine reacts with p-arsanilic acid to form a diazonium compound. This new diazonium compound changes colour to show if nitrates are present by coupling with 1 N-(1-naphthyl) ethylenediamine.
How do I use the Suresign UTI Test?
Before using the Suresign UTI test, it is important to follow these steps to ensure accurate results:
- The test is for in vitro use only (i.e. within a glass).
- Only collect urine using a clear container that is not contaminated by cleaning fluids.
- Follow the indicated times strictly.
- It is recommended to take a sample of urine for the test early in the morning as this is when urine is likely to be most concentrated.
- For women only: the test should not be performed during or for 3 days after your period.
To use the test:
- Collect urine in a clear glass or a plastic cup without any residual detergents.
- Open the foil pouch and take out the test trip. Make sure not to touch the test pads.
- Once the test pouch is opened, it is recommended to perform the test immediately.
- Dip the test strip in the urine sample.
- Hold the end of the test strip farthest away from the test pad and drip the test strip into the urine sample for 1-2 seconds, ensuring all test pads are fully immersed in the urine.
- Remove the strip and wipe off any surplus urine against the rim of the container to avoid mixing chemicals from adjacent reagent areas.
- Lay the strip with the test pads facing up on a flat surface and begin timing. Wait for 2 minutes (do not read the results after 3 minutes).
- Read the result separately for each parameter, and compare the colour with the colour chart provided in the insert found in the test kit packaging.
How do I interpret the test results?
The negative result includes:
- The test fields for Leukocytes stayed whitish.
- The test field for Nitrite stayed white.
The positive result for Leukocytes includes:
- The leukocyte test pad changed its colour to purple.
The positive result for Nitrite includes:
- The Nitrite test pad has changed to pink.